CD9 Antibody (NBP1-43893)(100tests)

Catalog number: NBP1-43893-100tests
Price: Ask price from support chat or by Email
Product name: CD9 Antibody (NBP1-43893)(100tests)

Applications

FACS
Uses:

This eBioSN4 (SN4 C3-3A2) antibody has been pre-titrated and tested by flow cytometric analysis of normal human platelets. This can be used at 20 μl (0.06 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test.

Dilutions: flow cytometry / FACS analysis

Background

The SN4 monoclonal antibody reacts with human CD9. CD9 is a 24 kDa member of the tetraspanin family, whose members are characterized by the presence of 4 hydrophobic transmembrane domains. CD9 is expressed in platelets, eosinophils, basophils, pre-B cells, activated T cells and neural cell lines. Furthermore, CD9 expression has been associated with a malignant phenotype, including expression on 90% of non T cell acute lymphoblastic leukemia cells and on 50% of chronic lymphocytic and acute myeloblastic leukemias. In platelets, CD9 is expressed in alpha-granules and through association with the integrin alphaIIb/betaIII plays a role in platelet aggregation, as demonstrated by the use of anti-human CD9 antibodies. CD9 has also been shown to induce the aggregation of pre-B cell lines, and the adhesion and migration of pre-B cells and Schwann cells. Additionally, it has been demonstrated that CD9 is able to provide a co-stimulatory signal for T cells independently of CD28, in the absence of antigen-presenting cells. Binding of the SN4 monoclonal antibody partially cross-blocks binding of another anti-human CD9 monoclonal antibody, MM2/57.

Details

Immunogen: The immunogen for this antibody was CD9.
Conjugate: PE
Clone: SN4 C3-3A2
Isotype: IgG1

Notes

Packaging

Storage: Store at 4C. Do not freeze.
Buffer: PBS [pH 7.2] containing BSA and/or gelatin for protein stabilization
Preservative: 0.09% Sodium Azide
Unit Size: 100tests (also available: )
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Publications

Species

Human
Reactivity: This antibody is reactive to Human..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Summary

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Summary:

Teste

FACS
Uses:

This eBioSN4 (SN4 C3-3A2) antibody has been pre-titrated and tested by flow cytometric analysis of normal human platelets. This can be used at 20 μl (0.06 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test.

Dilutions: flow cytometry / FACS analysis