IL2 Receptor alpha Antibody (NBP1-43880)(0.05mg)

Catalog number: NBP1-43880-005mg
Price: Ask price from support chat or by Email
Product name: IL2 Receptor alpha Antibody (NBP1-43880)(0.05mg)

Applications

FACS
Uses:

The PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocyte cell suspensions and ConA-activated splenocyte culture. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Dilutions: flow cytometry / FACS analysis

Background

The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Details

Immunogen: The immunogen for this antibody was PE CD25.
Conjugate: PE
Clone: PC61.5
Isotype: IgG1 Lambda

Notes

Packaging

Storage: Store at 4C. Do not freeze.
Buffer: Phosphate buffer pH 7.2,
Preservative: 0.09% Sodium Azide
Unit Size: 0.05mg (also available: , )
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Publications

Species

Mouse
Reactivity: This antibody is reactive to Mouse..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Summary

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Summary:

Teste

FACS
Uses:

The PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocyte cell suspensions and ConA-activated splenocyte culture. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Dilutions: flow cytometry / FACS analysis