Ctip1 Antibody (NB600-259)(0.1ml)

Catalog number: NB600-259
Price: Ask price from support chat or by Email
Product name: Ctip1 Antibody (NB600-259)(0.1ml)

Applications

WB, IP
Uses: Western Blot and Immunoprecipitation
Dilutions: immunoprecipitation 1 to 5 mcg/mg lysate,Western Blot 1:1000 - 1:10000

Background

The B cell leukemia 11A protein (BCL11A/Evi9/CTIP1) has been implicated in hematopoietic cell development and malignancies. BCL11A is a transcriptional repressor that binds directly to a GC-rich motif and is also recruited to a promoter template via interaction with the orphan nuclear receptor, chicken ovalbumin upstream promoter transcription factor II. In both cases, BCL11A-mediated transcriptional repression is only minimally reversed by trichostatin A, suggesting the possible lack of involvement of class I or II histone deacetylases. Nonetheless, chromatin immunoprecipitation assays revealed that expression of BCL11A in mammalian cells resulted in deacetylation of histones H3 and/or H4 that were associated with the promoter region of a reporter gene. BCL11A mediated transcriptional repression, as well as deacetylation of histone H3/H4 in BCL11A-transfected cells, was partially reversed by nicotinamide, an inhibitor of class III histone deacetylases such as SIRT1. SIRT1 was found to interact directly with BCL11A and was recruited to the promoter template in a BCL11A-dependent manner leading to transcriptional repression. These findings indicate a role for SIRT1 in transcriptional repression mediated by BCL11A in mammalian cells.

Details

Immunogen: The epitope recognized by this antibody maps to a region between residues 450 and 500 of human B-cell CLL/Lymphoma 11A using the numbering given in Swiss-Prot entry Q9H165 (GeneID 53335).

Notes

Packaging

Storage: Store at 4C. Do not freeze.
Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
Preservative: 0.09% Sodium Azide
Concentration: 1.0 mg/ml
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Publications

Species

Human
Reactivity: Cross reacts with Human. Reactivity with Mouse is expected due to complete identity between this ortholog and Human at the site to which the epitope maps..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Summary

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Summary:

Teste

WB, IP
Uses: Western Blot and Immunoprecipitation
Dilutions: immunoprecipitation 1 to 5 mcg/mg lysate,Western Blot 1:1000 - 1:10000