MHC class II Antibody (NBP1-43938)(0.1mg)

Catalog number: NBP1-43938-01mg
Price: Ask price from support chat or by Email
Product name: MHC class II Antibody (NBP1-43938)(0.1mg)

Applications

FACS
Uses:

The 14-4-4S antibody has been tested by flow cytometric analysis of mouse splenocyte suspension and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 �L. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Dilutions: flow cytometry / FACS analysis

Background

The 14-4-4S monoclonal antibody reacts with the mouse and rat major histocompatibility complex class II, I-E subregion-encoded glycoprotein in mice strains of H-2k, H-2d, H-2p and H-2r. H-2b, H-2f and H-2s haplotypes do not express the I-E antigen. MHC class II is present on B cells, monocytes, macrophages, and dendritic cells. The 14-4-4S mAb has been reported to block antigen presentation in in vitro assays. This antibody cross-reacts with rat.

Details

Immunogen: The immunogen for this antibody was MHC class II.
Clone: 14-4-4S
Isotype: IgG2a Kappa

Notes

Packaging

Storage: Store at 4C. Do not freeze.
Buffer: Phosphate buffer pH 7.2,
Preservative: 0.09% Sodium Azide
Unit Size: 0.1mg (also available: , )
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Publications

Species

Rat, Mouse
Reactivity: This antibody is reactive to Mouse, Rat..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Summary

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Summary:

Teste

FACS
Uses:

The 14-4-4S antibody has been tested by flow cytometric analysis of mouse splenocyte suspension and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 �L. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Dilutions: flow cytometry / FACS analysis