CCR7 Antibody (NBP1-43794)(25tests)

Catalog number: NBP1-43794
Price: Ask price from support chat or by Email
Product name: CCR7 Antibody (NBP1-43794)(25tests)

Applications

FACS
Uses:

This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.125 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test.

It is recommended that the staining incubation time be increased to at least 45 minutes at 4°C for optimal staining.

Dilutions: flow cytometry / FACS analysis

Background

The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of nave from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DCs. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.

Details

Immunogen: The immunogen for this antibody was CCR7.
Conjugate: PE
Clone: 3D12
Isotype: IgG2a Kappa

Notes

Packaging

Storage: Store at 4C. Do not freeze.
Buffer: PBS [pH 7.2] containing BSA and/or gelatin for protein stabilization
Preservative: 0.09% Sodium Azide
Unit Size: 25tests (also available: )
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Publications

Species

Human
Reactivity: This antibody is reactive to Human..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Summary

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Summary:

Teste

FACS
Uses:

This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.125 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test.

It is recommended that the staining incubation time be increased to at least 45 minutes at 4°C for optimal staining.

Dilutions: flow cytometry / FACS analysis