CD300E Antibody (NBP1-43786)(100tests)

Catalog number: NBP1-43786-100tests
Price: Ask price from support chat or by Email
Product name: CD300E Antibody (NBP1-43786)(100tests)

Applications

FACS
Uses: NBP1-43784

This UP-H2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μl (0.25 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test.

Dilutions: flow cytometry / FACS analysis

Background

The monoclonal antibody UP-H2 recognizes human IREM-2 (immune receptor expressed in monocytic derived cells) also known as LMIR6, and unofficially CD300E and CD300LE. It is a member of the CMRF-35 family. This 34 kDa Ig-like containing receptor is an activating receptor and through its charged residues in the cytokplasmic domain interacts with ITAM containing adaptor proteins like DAP12. Expression is found exclusively on monocytes and myeloid DCs. Expression may be transient as differentiation of monocytes leads to down regulation of IREM-2 yet expression is found on mature DCs. It has been also noted that myeloid cell lines such as THP-1 and U937 do not express IREM-2. In vitro addition of UP-H2 antibody either soluble or crosslinked stimulates monocytes.

Details

Immunogen: The immunogen for this antibody was IREM2.
Clone: UP-H2
Isotype: IgG1

Notes

Packaging

Storage: Store at 4C. Do not freeze.
Buffer: PBS [pH 7.2] containing BSA and/or gelatin for protein stabilization
Preservative: 0.09% Sodium Azide
Unit Size: 100tests (also available: )
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Publications

Species

Human
Reactivity: This antibody is reactive to Human..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Summary

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Summary:

Teste

FACS
Uses: NBP1-43784

This UP-H2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μl (0.25 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test.

Dilutions: flow cytometry / FACS analysis