CD99 Antibody (NB100-78009)(0.1mg)

Catalog number: NB100-78009
Price: Ask price from support chat or by Email
Product name: CD99 Antibody (NB100-78009)(0.1mg)

Applications

IHC, FACS
Uses: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. IMmunohistochemistry. It is recommended that the reagent be titrated for optimal performance for each application.
Dilutions: flow cytometry / FACS analysis immunohistochemistry

Background

The HCD99 monoclonal antibody recognizes human CD99 also known as CD99 antigen, E2 antigen, MIC2, and T-cell surface glycoprotein E2. CD99 is a type I, single chain transmembrane protein devoid of N-linked glycosylation sites that is encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, T cell leukemias and lymphomas and is absent on fetal B cells, some B cell lines, eosinophils, granulocytes and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin beta 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H. The HCD99 antibody has been reported to be useful for flow cytometric detection of human CD99 and immunohistochemistry (acetone-fixed frozen tissues and formalin-fixed paraffin-embedded tissues).

Details

Immunogen: The immunogen was the full length version of the protein: CD99 (HCD99)
Clone: HCD99
Isotype: IgG2a Kappa

Notes

Packaging

Storage: Store at 4C. Do not freeze.
Buffer: PBS [pH 7.2]
Preservative: 0.09% Sodium Azide
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Publications

  1. Gelin, C., et al. 1989. EMBO J. 8:3253.
  2. Goodfellow, P.J., et al. 1986. Science 234:740.
  3. Pettersen, R.D., et al. 2001. J. Immunol. 166:4931.

Species

Human
Reactivity: Human. .
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

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Summary:

Teste

IHC, FACS
Uses: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. IMmunohistochemistry. It is recommended that the reagent be titrated for optimal performance for each application.
Dilutions: flow cytometry / FACS analysis immunohistochemistry