OCT4 Antibody (NBP1-43758)(0.1mg)

Catalog number: NBP1-43758-01mg
Price: Ask price from support chat or by Email
Product name: OCT4 Antibody (NBP1-43758)(0.1mg)

Applications

FACS
Uses:

This EM92 antibody has been tested by intracellular staining and flow cytometric analysis of F9 embryonal carcinoma cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Staining should be carried out using the eBioscience Foxp3 Staining Buffer Set (00-5523).

Dilutions: flow cytometry / FACS analysis

Background

The EM92 monoclonal antibody reacts with mouse and human Oct-3/4, encoded by the Pou5F1 gene. Oct-3/4 is a POU domain-containing transcription factor that is critical for maintaining embryonic stem (ES) cells in a pluripotent state, and is expressed by ES, embryonic germ cells and embryonic carcinoma cell lines. In cells of the inner cell mass (ICM), reduction of Oct-3/4 expression causes dedifferentiation to trophoectoderm, whereas increased expression results in differentiation to mesoderm and primitive endoderm. Oct-3/4 regulates the expression of several genes, including FGF-4, UTF1, Sox2, Fbx15, Rex1 and osteopontin through distinct mechanisms. Furthermore, Oct-3/4 frequently acts synergistically with Sox2 to regulate target gene expression, as is the case with FGF-4. It has been demonstrated that Oct-3/4 expression in ES cells can be negatively regulated by either treatment with retinoic acid, or by removal of leukemia-inhibitory factor (LIF).

Details

Immunogen: The immunogen for this antibody was OCT34, Pou5f1, transcription factor 1.
Clone: EM92
Isotype: IgG2a Kappa

Notes

Packaging

Storage: Store at 4C. Do not freeze.
Buffer: PBS [pH 7.2]
Preservative: 0.09% Sodium Azide
Unit Size: 0.1mg (also available: )
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Publications

Species

Mouse
Reactivity: This antibody is reactive to Mouse..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Summary

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Summary:

Teste

FACS
Uses:

This EM92 antibody has been tested by intracellular staining and flow cytometric analysis of F9 embryonal carcinoma cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Staining should be carried out using the eBioscience Foxp3 Staining Buffer Set (00-5523).

Dilutions: flow cytometry / FACS analysis