CD244 Antibody (NBP1-43759)(25tests)

Catalog number: NBP1-43759-25tests
Price: Ask price from support chat or by Email
Product name: CD244 Antibody (NBP1-43759)(25tests)

Applications

FACS
Uses:

This eBioC1.7 (C1.7) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 20 μl (0.125 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test.

Dilutions: flow cytometry / FACS analysis

Background

The C1.7 monoclonal antibody reacts with human CD244 (2B4, p38). In human, CD244 is a 38 kDa protein expressed on NK cells, a subset of CD8+ T cells, gammadelta T cells, monocytes, basophils and eosinophils. Binding of the CD244 ligand, CD48, results in NK cell activation, unlike mouse CD244, which is an inhibitory receptor. For CD244 expressed on NK cells, binding of CD48 results in enhanced NK cell cytotoxicity and secretion of IFN-gamma. Recently, it was demonstrated that binding of the C1.7 monoclonal antibody and CD48 involve the same residue in the V domain of human CD244, which explains the ability of C1.7 binding to induce activation of NK cells. Binding of C1.7 to CD244 leads to tyrosine phosphorylation and recruitment of the adaptor molecule SAP (SLAM-associated protein). Patients with X-linked lymphoproliferative disorder (XLPD) have a mutation in SAP which renders it unable to bind to phosphorylated CD244.

Details

Immunogen: The immunogen for this antibody was CD244.
Clone: C1.7 (C1.7)
Isotype: IgG1 Kappa

Notes

Packaging

Storage: Store at 4C. Do not freeze.
Buffer: PBS [pH 7.2] containing BSA and/or gelatin for protein stabilization
Preservative: 0.09% Sodium Azide
Unit Size: 25tests (also available: )
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Publications

Species

Human
Reactivity: This antibody is reactive to Human..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Summary

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Summary:

Teste

FACS
Uses:

This eBioC1.7 (C1.7) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 20 μl (0.125 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test.

Dilutions: flow cytometry / FACS analysis