IL17 Antibody (NBP1-43721)(100tests)

Catalog number: NBP1-43721-100tests
Price: Ask price from support chat or by Email
Product name: IL17 Antibody (NBP1-43721)(100tests)

Applications

FACS
Uses:

The fluorochrome-conjugated eBio64CAP17 antibody has been tested by intracellular staining for flow cytometric analysis and can be used at less than or equal to 0.25 ug (mass format) per million cells in 100 ul staining volume. Please refer to the antibody vial for the recommended test volume to use for one million cells in a 100 μl total staining volume.

The Affinity Purified eBio64CAP17 antibody has been tested as the capture antibody in a sandwich ELISA for measurement of human IL-17A protein levels, in combination with the biotinylated eBio64DEC17 antibody (13-7179) for detection and recombinant human IL-17A (14-8179) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1.0 - 4.0 ug ug/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.

The Functional Grade Purified eBio64CAP17 antibody has been tested by LAL assay to verify low endotoxin and has been tested for neutralization of IL-17A bioactivity and for ELISPOT capture.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Dilutions: flow cytometry / FACS analysis

Background

The 64CAP17 antibody reacts with human IL-17A the antibody has been reported to cross react with Rhesus monkey IL-17A, as verified by intracellular staining experiments. The 64CAP17 antibody is a neutralizing antibody. Reactivity of the 64CAP17 antibody with other IL-17 family members has not been evaluated. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor. IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells. Additionally, activated human CD4+ T cells have been found to produce the IL-17A/F heterodimer, as well as the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-a secretion IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). 64CAP17 can be used to detect IL-17 heterodimers by immunoprecipitation followed by immunoblot with anti-IL17F monoclonal antibody. The 64CAP17 has been shown to react to rhesus and marmoset primates.

Details

Immunogen: The immunogen for this antibody was IL17A.
Clone: 64CAP17
Isotype: IgG1 Kappa

Notes

Packaging

Storage: Store at 4C. Do not freeze.
Buffer: Phosphate buffer pH 7.2,
Preservative: 0.09% Sodium Azide
Unit Size: 100tests (also available: )
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Publications

Species

Human
Reactivity: This antibody is reactive to Human..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Summary

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Summary:

Teste

FACS
Uses:

The fluorochrome-conjugated eBio64CAP17 antibody has been tested by intracellular staining for flow cytometric analysis and can be used at less than or equal to 0.25 ug (mass format) per million cells in 100 ul staining volume. Please refer to the antibody vial for the recommended test volume to use for one million cells in a 100 μl total staining volume.

The Affinity Purified eBio64CAP17 antibody has been tested as the capture antibody in a sandwich ELISA for measurement of human IL-17A protein levels, in combination with the biotinylated eBio64DEC17 antibody (13-7179) for detection and recombinant human IL-17A (14-8179) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1.0 - 4.0 ug ug/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.

The Functional Grade Purified eBio64CAP17 antibody has been tested by LAL assay to verify low endotoxin and has been tested for neutralization of IL-17A bioactivity and for ELISPOT capture.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Dilutions: flow cytometry / FACS analysis