MHC Class I Antibody (NBP1-43705)(25tests)

Catalog number: NBP1-43705
Price: Ask price from support chat or by Email
Product name: MHC Class I Antibody (NBP1-43705)(25tests)

Applications

FACS
Uses:

This 3D12 (HLA-E) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μl (0.5 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test.

Dilutions: flow cytometry / FACS analysis

Background

The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.

Details

Immunogen: The immunogen for this antibody was HLAE.
Clone: 3D12HLA-E
Isotype: IgG1

Notes

Packaging

Storage: Store at 4C. Do not freeze.
Buffer: PBS [pH 7.2] containing BSA and/or gelatin for protein stabilization
Preservative: 0.09% Sodium Azide
Unit Size: 25tests (also available: )
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Publications

Species

Human
Reactivity: This antibody is reactive to Human..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Summary

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Summary:

Teste

FACS
Uses:

This 3D12 (HLA-E) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μl (0.5 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test.

Dilutions: flow cytometry / FACS analysis