LYVE1 Antibody (NBP1-43645)(0.1mg)

Catalog number: NBP1-43645-01mg
Price: Ask price from support chat or by Email
Product name: LYVE1 Antibody (NBP1-43645)(0.1mg)

Applications

IHC-Fr, FACS
Uses:

This ALY7 antibody has been tested by flow cytometric analysis of LYVE-1/GFP co-transfected cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Dilutions: flow cytometry / FACS analysis, Immunohistochemistry-Frozen

Background

The monoclonal antibody ALY7 recognizes mouse LYVE-1, a transmembrane glycoprotein with similarity to CD44. The extracellular domain contains a conserved hyaluronan binding domain also found in CD44. Expression is found on lymphatic and liver endothelial cells and some populations of macrophages. The lymphatic system is responsible for transporting proteins and cells (especially dendritic cells) to tissues throughout the body, thereby acting as immune surveyors. LYVE-1 is one characteristic protein, along with podoplanin, PROX-1, Tie-2 and VEGFR-3, that is expressed on lymphatic endothelial cells (LECS). The ligand for LYVE-1 is hyaluronan, a large mucopolysaccharide. Although LYVE-1 can bind hyaluronan in vitro, the site for ligand binding in vivo is masked by sialyated O-linked glycan chains. It is postulated that binding to ligand requires modification/unmasking to expose the binding site. The development and remodeling of the endothelium after injury is an area of extensive study. When transplanted, hematopoietic stem cells (HSCs) can give rise to LECs that integrate into the endothelium in normal and metastatic tissue.

Details

Immunogen: The immunogen for this antibody was LYVE1.
Conjugate: Biotin
Clone: ALY7
Isotype: IgG1

Notes

Packaging

Storage: Store at 4C. Do not freeze.
Buffer: Aqueous buffer (contains stabilizer if necessary)
Preservative: 0.09% Sodium Azide
Unit Size: 0.1mg (also available: )
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Publications

Species

Mouse
Reactivity: This antibody is reactive to Mouse..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Summary

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Summary:

Teste

IHC-Fr, FACS
Uses:

This ALY7 antibody has been tested by flow cytometric analysis of LYVE-1/GFP co-transfected cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Dilutions: flow cytometry / FACS analysis, Immunohistochemistry-Frozen