TNF Receptor II Antibody (NBP1-43588)(0.5mg)

Catalog number: NBP1-43588-05mg
Price: Ask price from support chat or by Email
Product name: TNF Receptor II Antibody (NBP1-43588)(0.5mg)

Applications

ELISA, FACS
Uses:

The TR75-32 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse TNFRII in combination with the purified TR75-54 antibody for capture and recombinant mouse TNFRII as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 ug/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

Dilutions: flow cytometry / FACS analysis, ELISA

Background

The TR75-32 monoclonal antibody reacts with mouse Tumor Necrosis Factor Receptor II (TNFRII, TNFR-p80, TNFRSF1B, CD120b). TNFRII is expressed in a variety of cell types and strongly expressed on stimulated T and B lymphocytes. TNFRII is the main TNF receptor found on circulating T cells and is the major mediator of autoregulatory apoptosis in CD8+ cells. The soluble TNF receptors are truncated forms of cell surface receptors with neutralizing activity on both TNF-a and TNF-. It has been suggested that the proinflammatory and immunosuppressive properties of TNF segregate at the level of its receptors and that the pool of TNF bound to soluble receptors could represent a reservoir to control the TNF release.

Details

Immunogen: The immunogen for this antibody was TNFR2p75.
Conjugate: Biotin
Clone: TR75-32
Isotype: IgG

Notes

Packaging

Storage: Store at 4C. Do not freeze.
Buffer: PBS [pH 7.2]
Preservative: 0.09% Sodium Azide
Unit Size: 0.5mg (also available: )
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Publications

Species

Mouse
Reactivity: This antibody is reactive to Mouse..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Summary

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Summary:

Teste

ELISA, FACS
Uses:

The TR75-32 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse TNFRII in combination with the purified TR75-54 antibody for capture and recombinant mouse TNFRII as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 ug/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

Dilutions: flow cytometry / FACS analysis, ELISA