FOXP3 Antibody (NBP1-43556)(0.1mg)

Catalog number: NBP1-43556-01mg
Price: Ask price from support chat or by Email
Product name: FOXP3 Antibody (NBP1-43556)(0.1mg)

Applications

IHC-P, IHC-Fr, FACS
Uses:

This 236A/E7 antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood mononuclear cells using the Foxp3 Staining Buffers. This can be used at less than or equal to 0.25 ug per 1 million cells in 100 ul total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Dilutions: flow cytometry / FACS analysis, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Background

science offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3+ cells. Other antibodies to human Foxp3 include the recently published PCH101 and 7979 . The 236A/E7 antibody reacts with human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2 cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in scurfy (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3 Staining Buffer Set and protocol reveals staining of the CD4+CD25bright population. The epitope from 236A/E7 is different from that of PCH101 .

Details

Immunogen: The immunogen for this antibody was Foxp3.
Conjugate: Biotin
Clone: 236A/E7
Isotype: IgG1

Notes

Packaging

Storage: Store at 4C. Do not freeze.
Buffer: Phosphate buffer pH 7.2,
Preservative: 0.09% Sodium Azide
Unit Size: 0.1mg (also available: )
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Publications

Species

Human
Reactivity: This antibody is reactive to Human..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Summary

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Summary:

Teste

IHC-P, IHC-Fr, FACS
Uses:

This 236A/E7 antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood mononuclear cells using the Foxp3 Staining Buffers. This can be used at less than or equal to 0.25 ug per 1 million cells in 100 ul total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Dilutions: flow cytometry / FACS analysis, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin