MHC Class II Antibody (NBP1-43548)(0.1mg)

Catalog number: NBP1-43548
Price: Ask price from support chat or by Email
Product name: MHC Class II Antibody (NBP1-43548)(0.1mg)

Applications

FACS
Uses:

This eBioY-Ae (YAe, Y-Ae) antibody has been tested by flow cytometric analysis of a mouse strain known to express the I-A(b)/MHC Class II:Ea peptide complex. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Dilutions: flow cytometry / FACS analysis

Background

The Y-Ae antibody reacts with a class II major histocompatibility complex (MHC) self Ea peptide (peptide 52-68) bound to I-Ab molecules. The Y-Ae antibody detects a determinant expressed on a subset of class II I-Ab molecules in strains that also express class II I-Eb. This determinant is expressed on peripheral B cells and on cells in the thymic medulla, but not thymic cortical epithelium. In addition to B cells, the Y-Ae determinant is expressed at comparable levels on other antigen presenting cells, including macrophages and dendritic cells. The antibody does not react with invariant chain-associated class II MHC complexes. The reactivity of the Y-Ae antibody is MHC-restricted. The Y-Ae antibody was generated by immunization of [B10.MBR x B10.D2]F1 recipients with B10A(5R) LPS blasts. In this strain combination, there is incompatibility for I-Ab and I-Eb molecules. The antibody reacts with 10-15% of surface I-Ab molecules in strains that also express surface I-Eb molecules (e.g., strains B10.A(5R) and B10.A(3R)). The antibody does not react with I-A molecules in strains that have a non-functional I-E (a chain) gene (e.g., strains B6 and B10), nor with mutant I-A molecules (e.g., (B6.c-H-2bm-12 x A.TFR5)F1), nor with any other strain tested. The Y-Ae antibody recognizes an Ea peptide (Ea 52-68) bound to I-Ab molecules, but not to Ea 52-68 peptide bound to I-Ak or I-Abm-12 molecules.

Details

Immunogen: The immunogen for this antibody was Ea5268 peptide bound to the IA(b)MHC Class II.
Conjugate: Biotin
Clone: Y-Ae (YAe, Y-Ae)
Isotype: IgG2b

Notes

Packaging

Storage: Store at 4C. Do not freeze.
Buffer: PBS [pH 7.2]
Preservative: 0.09% Sodium Azide
Unit Size: 0.1mg (also available: , )
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Publications

Species

Mouse
Reactivity: This antibody is reactive to Mouse..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Summary

Labosample Reward Points:
  • Review this product for a reward and claim prizes ()
  • 100 points for each review and 100 for an image
  • We value both positive and negative feedback!
  • Reviews can be done at
(image) Labosample’ Quality Guarantee
We stand behind our products 100%. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Labosample’ Quality Guarantee covers 100% of the products we carry, including those that we distribute for Abnova. No hassles, no nonsense. It is that simple!

+ Innovator’s Reward™
Our Innovator’s Reward™ program is designed to support your innovative research with minimal financial risk to you. Should you decide to use one of our products in an application or species for which it has not been tested, Labosample will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. Simply email info@gentaur.com to apply for your award. We will send you a form for you to submit a summary of your research and report your experimental results, after which your award will be given. Your innovation can help us to accelerate bioscience research. After all, we’re making your success our goal.

= 100% Support and Peace of Mind


Summary:

Teste

FACS
Uses:

This eBioY-Ae (YAe, Y-Ae) antibody has been tested by flow cytometric analysis of a mouse strain known to express the I-A(b)/MHC Class II:Ea peptide complex. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Dilutions: flow cytometry / FACS analysis